Samples for NIPT and RhD test

Peripheral blood sampling for NIPT and RhD test

The NIPT (Non-Invasive Prenatal Test) and the Non-Invasive Foetal Resus-D Determination (RhD test) are based on the examination of the total (maternal + foetal) fraction of cell-free DNA in maternal plasma. It was shown that during pregnancy DNA originating from the placenta (placenta) enters the maternal bloodstream. This cell-free foetal DNA (cfDNA) is fragmented and is only a fraction of the total cell-free DNA present in maternal plasma.
The aim is to stabilise the cell-free DNA and to avoid cell degradation of maternal cells, leading to the release of genomic maternal DNA.
For the non-invasive prenatal test (NIPT), and/or in combination with the RhD test, 10 ml of peripheral blood should be collected from the mother during pregnancy (from 12 weeks) with a 21G needle. Please do not use a needle with a fine lumen such as a wing needle; cells may be damaged by the smaller lumen.
Mark the blood tube with the patient's identity information.
The blood is preferably collected in a PAXgene blood tube. This is because this blood tube stabilises the cell-free DNA in the mother's blood. If this type of tube is not available, an EDTA tube can be used.
Please gently invert the blood tube several times after collection so that the blood is well mixed.
Then put the blood tube in a safety tube (labeled "NIPT" on it).
How to store: at room temperature, shielded from bright sunlight
Delivery to the laboratory: to be delivered with the request form, within 24 hours of collection, before 5 pm (on Fridays before 2 pm) (internally this may be done by tube mail: 2477)

Contact the laboratory for your questions or in case of a problem: 09-332 24 77.

Last updated: 07 December 2023 - 14:12
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