Center for Medical Genetics Center for Medical Genetics

Constitutional - DNA

Postnatal samples for DNA tests

Blood

instructions for the sampling of blood and bone marrow for DNA tests

Cheek brush

Instructions for buccal cell sample collection

Fresh human tissue other than blood

Formalin-fixed paraffin-embedded tissue

For DNA tests (f.e. the detection of somatic mutation in tumour tissue) it is crucial that the tissue has been fixed with fresh buffered formalin (10% formalin of neutral pH; formalin of too low pH can cause shearing of the DNA strands) and that a fixation duration of 6 to 72 hours is respected. The time between the ressection of the tissue and the fixation must be as short as possible. Reference: Asco guidelines: Wolff et al. J. Clin. Oncol. 2013, 31: 3997-4013.

DNA

instructions for the sampling of blood and bone marrow for DNA tests

Preferably, we get an EDTA blood sample. If you have the patient’s DNA, the following conditions are appropriate for almost any molecular-genetic test:

Fresh blood (indicated as ‘f-E’ on the application form)

instructions for the sampling of blood and bone marrow for DNA tests

BIOCHEMICAL TESTS AND NULL-ALLELE TESTING

Fibroblasts

Skin biopsy



Last updated: 07 November 2017 - 17:49
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